GLP-3 specifications and molecular characteristics
The technical specification for GLP-3 RT, the GLP-3 research material supplied by GLP-3 Research Supply: molecular formula C221H342N46O68, average molecular weight 4731.33 g/mol, 39 residues, supplied as a white lyophilized powder. Each item is explained below.
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Specification table
| Product | GLP-3 RT |
|---|---|
| Type | Synthetic research peptide |
| Molecular formula | C221H342N46O68 |
| Molecular weight | 4731.33 g/mol (average) |
| Length | 39 residues |
| Sequence | Tyr-Aib-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Ile-αMeLeu-Leu-Asp-Lys-[Lys(C20 diacid-γGlu-AEEA)]-Ala-Gln-Aib-Ala-Phe-Ile-Glu-Tyr-Leu-Leu-Glu-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2 |
| N-terminus | Free amine (Tyr1) |
| C-terminus | Amide (Ser39-NH2) |
| Non-standard residues | Aib (2, 20), α-methyl-leucine (13) |
| Side-chain modification | Lys17: C20 fatty diacid via γGlu-AEEA |
| Receptor design | GIP receptor (GIPR), GLP-1 receptor (GLP-1R) and glucagon receptor (GCGR) |
| Form | White lyophilized powder |
| Vial sizes | 10 mg, 15 mg, 30 mg |
| Purity specification | ≥95% (HPLC-UV) |
| Purity, current lots | 99.67%–99.98% |
| Identity specification | Confirmed by LC-MS |
| Testing laboratory | Freedom Diagnostics (independent) |
| Storage | Store sealed, dry and protected from light at the temperature stated on the vial label. Allow a cold vial to reach room temperature before opening. |
| Designation | Research use only |
Current lot results against specification
Every current lot meets the release specification. Current lots test at 99.67%–99.98% by HPLC-UV against a specification of ≥95%, and identity is confirmed by LC-MS on every lot.
GLP-3 molecular formula
The molecular formula of GLP-3 RT is C221H342N46O68. The formula includes the 39 amino acid residues, the C-terminal amide and the lipid side chain on lysine 17.
GLP-3 molecular weight
The average molecular weight of GLP-3 RT is 4731.33 g/mol. "Average" means it is calculated from natural isotope abundances, which is the value used to predict the ions seen in LC-MS. With this weight, GLP-3 RT is expected at m/z 1578.1 with three charges and m/z 1183.8 with four charges. Current certificates show 1578.0–1578.3 and 1183.6. How identity is confirmed.
GLP-3 sequence
Tyr-Aib-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Ile-αMeLeu-Leu-Asp-Lys-[Lys(C20 diacid-γGlu-AEEA)]-Ala-Gln-Aib-Ala-Phe-Ile-Glu-Tyr-Leu-Leu-Glu-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2
The sequence is written N-terminus to C-terminus. Positions 2 and 20 carry α-aminoisobutyric acid (Aib) and position 13 carries α-methyl-leucine; both add a methyl group to the α-carbon and constrain the backbone. The C-terminal serine is amidated.
Structure and modifications
Two α-aminoisobutyric acid (Aib) residues and one α-methyl-leucine constrain the peptide backbone. A C20 fatty diacid, attached to lysine 17 through a γ-glutamate and AEEA spacer, makes the molecule strongly hydrophobic, so it elutes late on reversed-phase HPLC. The C-terminus is amidated. The sequence contains no methionine, cysteine, tryptophan, histidine or asparagine.
Full molecular characteristics.
Receptor classification
GLP-3 RT is designed for activity at the GIP receptor (GIPR), GLP-1 receptor (GLP-1R) and glucagon receptor (GCGR). All three are class B G protein-coupled receptors.
Physical form
White lyophilized powder, recorded as the appearance on every certificate. Lyophilization (freeze-drying) removes water, which slows most chemical degradation. What lyophilized means.
Purity and identity specifications
GLP-3 Research Supply’s stated release specification is ≥95% HPLC-UV purity with identity confirmed by LC-MS. The current lot, KB89232, reported 99.98%. How purity is measured, or how specifications fit into the quality and supply-chain process.
Analytical and testing methods
HPLC-UV for purity, LC-MS for identity and laboratory quantitation for net content, performed on every lot by Freedom Diagnostics. LAL endotoxin testing (USP <85>) and PCR microbial screening are reported for lots where they were run. Testing and quality control.
Research designation
GLP-3 RT is designated research use only: supplied for laboratory research by qualified personnel. Research use only policy.
Buy GLP-3
10 mg, 15 mg and 30 mg vials. Current lot KB89232 tested at 99.98% purity with identity confirmed by LC-MS. Current purchasing is available through Klow.bio.